Expression of housekeeping genes varies depending on mevalonate pathway inhibition in cancer cells

Statins have anticancer effects and may be used as agents via drug repositioning. In reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays, the internal reference gene must not affected by any experimental conditions. As statins exert a wide range of on cells inhibiting mevalonate pathway, it is possible that statin treatment might alter expression housekeeping genes genes, thereby misleading assessment obtained data. Here, we evaluated stability in atorvastatin-treated cancer cell lines. We treated both statin-sensitive statin-resistant lines with atorvastatin at seven different concentrations performed RT-qPCR 15 whose was then assessed using five algorithms. lines, certain dose-dependent line-dependent manner; therefore, caution should exercised when comparing target between cells. Our findings emphasize importance validation analyses treatment-based research.